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For initial isolates from 2 women, MLCs of metronidazole were >12.5 µg/mL (50 µg/mL in both cases).
Initial isolates from blood cultures of an index patient and two laboratory workers were incorrectly identified as contaminants, in part because of reporting of primary Gram stain results as gram-positive and gram-variable.
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Only the initial isolate from each patient was counted and underwent molecular typing.
Nore1 was discovered in a two-hybrid screen using H-RasG12V as bait; the initial isolate, obtained from a murine T-cell cDNA library, was a partial cDNA encoding the Nore1B isoform, however, the isoform first characterized was a full-length murine Nore1A isolated from brain cDNA [15].
From 18 initial isolates, 9 distinct STs were identified; ST266 was found at 4 separate sites and ST109 at 3 (Table).
All 16 initial isolates (received October 26, 2010) were from Artibonite Department, the source of the outbreak in Haiti, and demonstrated little PFGE diversity.
Only initial isolates were included in the study; repeated isolates from the same patient were excluded.
In contrast, MLVA 97 strains were isolated from Tembladero Slough nearly two months after its initial isolation from the Gabilan.
During the 12-year study period, there were 89 C. neoformans initial clinical isolates from patients with invasive cryptococcosis, including 30 (34%%) non-susceptible to fluconazole.
After this isolate was identified, two other NDM-1-producing P. aeruginosa isolates with antibiograms identical to the initial isolate were cultured from the sputum samples of two other patients in April and May 2015.
Using this strategy, MAT a strain YO528 was derived from initial isolate EC-33.
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