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Conversely, the biomass productivity gradually decreased when the initial inoculum density increased to a certain degree.
At a high initial inoculum density, the hydrocarbon content slightly decreased.
The initial inoculum density was approximately 8 10 g m−2.
D5 and D12 reduced at least 1 log10 CFU/mL (90%) of the initial inoculum after 24 h.
Inactivation of three Saccharomyces cerevisiae strains by PEF treatment, at different growth phases and initial inoculum sizes was investigated.
Cultural conditions: P-Glycerol, 20%; dextrose, 0.5%; Initial inoculum, ~3 log CFU/ml; pH 7.0; Temperature, 37°C.
Fig. 2 Fermentation characteristics of strains in SECS hydrolysate with different sources of nitrogen and initial inoculum sizes.
Therefore, the control of initial inoculum density was a key to ensuring normal algal culture and metabolite generation.
In Fig. 2, the biomass productivity of B. braunii SAG 807-1 gradually increased as the initial inoculum density increased.
Typically, two indistinct zones develop at about 6 mm and 15 mm from the initial inoculum spot, with each zone roughly 4 mm wide.
The initial inoculum was approximately 108 cfu/ml.
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