At the end of both laboratory experiments, all leaves on these plants were carefully checked for infection and only the leaves that were initially inoculated were confirmed to support infection demonstrating that the initial inoculation had infected only the target leaf as intended.
In contrast, levels of LAB inoculated on the fresh-cut onions remained at the initial inoculation level of 5 × 105 cfu g-1 over the 12 d storage at 5°C (Figure 4b). Figure 4 The viability of L. innocua (a) and LAB (b) inoculated onto fresh-cut onions during 12 d storage at 5°C.
The harvested cultures were clarified by centrifugation, and the supernatants were inoculated onto fresh MDCK cells as described for the initial inoculation; up to 3 additional passages were performed for samples that did not show evidence of influenza virus by hemagglutination or RT-PCR.
Measurements were made from the initial inoculation until 18.5 days.
The initial inoculation density was maintained same for all the three systems in both the studies.
The appropriate range of initial inoculation density was 7.9 10.1 g m−2.
The mLAMP allowed the detection of milk sample artificially contaminated by Salmonella strains and Shigella strains at initial inoculation levels of approximate 5 CFU/10 mL.
All animals were given booster vaccination at day 21 after initial inoculation and guinea pigs were challenged 3 weeks after booster vaccination.
In contrast, Ph. murrayi (CAWBG22) increased in bulk at the initial inoculation site, with very little spread of filaments across the bottom of the culture bottle.
Five days after inoculation, both Ph. autumnale (CAWBG26) and Planktothrix sp. (CAWBG59) had filament growth extending from the initial inoculation site across the walls of the culture bottles.
In this study, seed age influenced the biomass of and hydrocarbon accumulation in B. braunii in the attached culture, and the initial inoculation density played important roles in B. braunii's growth.
