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These results provide initial harmonization guidelines to optimize Elispot assay performance to the immunotherapy community.
As a results of the first two Elispot proficiency panels, initial harmonization guidelines for Elispot [ 10] were developed.
Hence, there was a uniform criterion applied to all dot plots even though a subjective definition was used.> -wrap-foot> tableable shows the summary of the distribution of the number of times a lab was considered of having detected a response One aim of the CVC multimer panel was to deduce recommendations for initial harmonization guidelines of the assay.
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Based on the results of this multimer panel, we outline initial assay harmonization guidelines addressing four areas of immunomonitoring by HLA-peptide multimers (Table 5).
The outcomes from these two proficiency panels first provide Initial Elispot Harmonization Guidelines for Optimizing Assay Performance (Fig. 1) that can fulfill this need and may provide if implemented widely the grounds for substantial improvement of assay utility for research applications and development of immune therapies.
A global harmonization initiative is needed.
Harmonization initiatives have brought together individuals with diverse expertise.
As described below, our initial studies evaluated the utility of this codon harmonization algorithm by focusing on this region of the gene because, of all of the putative regions of slowly translated mRNA that were identified, this was the most discordant in its codon usage pattern.
But judicial harmonization cannot achieve a fully harmonized system.
Foursquare is launching an initiative that they're calling the "Venues Project" or "venue harmonization coordination".
A code book (harmonization guidelines) was made explaining how the variables were harmonized (see additional file 1: Harmonization guidelines).
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