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The steady-state luminescence is reached after an initial decay that last up to 300 s.
These plots show an initial decay that eventually reaches a plateau.
These plots show an initial decay that eventually reaches a plateau are relative short compared to the total imaging time (∼20 mins).
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More importantly, the Fbxl7 steady-state levels remain detectable with some degree of stabilization following its initial rapid decay that appears to be caused by the accelerated degradation of Fbxl18 based on our observations (data not shown).
This revealed that laser abscission of the lamellae leads to an initial rapid exponential decay that is caused by the sudden release of lamellar tension, followed by a slower linear phase as the retrograde actin flow continuously pulls in the network.
After data processing (subtraction of singly spin-labeled background and normalization), the depth of the initial decays indicated that each pair was almost fully labeled.
An analysis of the spatial variation of initial decay rate showed that an increase in the number of modes per band, and light and moderate damping can reduce the spatial variation.
A semi-quantitative analysis of initial decay rates showed that the complex I-inhibition-induced mitochondrial depolarization rates were as follows: control cells (1.44±0.03)×min−1; shRNALow cells (0.28±0.03)×min−1; and shRNAHigh cells (0.10±0.02)×min−1.
Smoke stacks, urban decay, that steaming asphalt.
It is concluded that the initial decay slopes can sometimes be determined within a very short decay interval (e.g., less than 10 dB) provided that the corresponding time interval is larger or much larger than the energy mean free path time.
Additionally, the effect of the direct field on the initial decay can be ignored provided that the response point is far enough from the drive point.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com