The initial data collection was carried out before the start of the intervention (during two weeks in July 2014).
The initial data collection was undertaken for 7 days from midnight on 1 March until midnight on 8 March 2010.
This surveillance system was rapidly established, and the initial data collection was retrospective from the medical record where symptoms were not always well documented.
This surveillance system was rapidly established, and initial data collection was retrospective from the medical record where symptoms were not always well documented.
Initial data collection was staggered over 7 months to allow for issues and findings arising during the early phases of data collection to inform, if necessary, the areas explored in later stages of data collection, in line with an inductive thematic approach.
This may be how factors such as depression or workload at initial data collection are risk factors for the onset of back pain at follow-up (e.g. [ 5, 46]), or how factors such as pain levels or psychological distress around the time of consultation predict poorer prognosis at follow-up (e.g. [ 47, 48]).
After optimization of the initial crystallization condition, crystals for data collection were grown using 100 mM HEPES (pH 7.2) or 100 mM sodium acetate (pH 4.4), 14% (w/v) PEG 8000, 160 mM calcium acetate and 20% (v/v) glycerol.
After the initial clinical examination, further waves of data collection were performed in 1989 (phase 2), 1991 1993 (phase 3, including a clinical examination), 1995 (phase 4), 1997 1999 (phase 5, including a clinical examination), 2001 (phase 6), and 2002 2004 (phase 7, including a clinical examination).
The median time to study enrollment and initial symptom data collection was 3 days [IQR 2, 3] following PEP initiation.
The initial microCT data collection was performed at 70 kVp and 114 μA with a resolution of 36 μm.
