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The result that pellets entirely cultured at 19% O2 negatively stained for type II collagen fragments could be explained by the insufficient accumulation of the MMP substrate (that is, type II collagen) during the initial cultivation Phase I.
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The initial cultivation terminated when all glycerol was consumed (about 14 h) at batch phase.
The main environmental impact is due to the cultivation phase while the transformation has limited consequences.
Initial cultivation was performed on tryptone soya agar (Oxoid, USA) for 18 h at 37°C.
This trait might evolve under initial cultivation and be regarded as part of 'semi-domestication'.
Rooted plantlets were greenhouse acclimatized by initial cultivation on 1/20th strength Hoagland and Arnon [ 126] nutrient solution.
The OSP supplementation considerably responded to different cultivation phases.
Changes in hyphal growth and morphology were analyzed at distinct cultivation phases using automated image analysis.
For the other cultivation phases, the C-balances did not close to 1.0.
Expression levels of actA decreased considerably after exponential growth but remained constant during later cultivation phases.
Cultures were run in batch mode and after an initial growth phase, cultivation temperature and pH were shifted.
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