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Such changes are believed to have influenced and even stimulated the initial cultivation and domestication of crop plants, as well as the domestication and pastoralization of animals.
And if one third of the initial cultivation area is still left, that means that a significant amount of cocaine is still coming out of Colombia, and will be for the foreseeable future.
The scientists found that, in the case of some crops, it would take several centuries of growing them to pay off the "carbon debt" caused by their initial cultivation.
A successive growth period showed same trends in the distribution of Cry-protein but on a higher level, as was related to a background concentration of Cry-proteins, resulting from the initial cultivation of transgenic rice cultivars.
Biohydrogen production by dark fermentation in a series of batch tests under different environmental control conditions was evaluated to determine the optimal initial cultivation pH and temperature for a continuous-flow kinetic test to validate the kinetic model system.
After incubated for 72 h under the optimized culture medium in 5-L bioreactor, the gellan gum fermentation reached about 19.90 ± 0.68 g/L, which was higher than that in the initial cultivation medium.
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The initial batch cultivation (duration about 30 h) was followed by approximately 50 h of continuous cultivation.
Cultivations were performed in 3-liter fermentors (Applikon) with an initial culture volume of 1.8 liter.
Cultivations were performed in 3-liter fermentors (Applikon) with an initial culture volume of 1 L or 1.8 L.
For both shake-flask and bioreactor fed-batch cultivations (initial batch culture), SD medium was supplemented with glucose to a final concentration of 5 g L−1 (j).
Cultures were run in batch mode and after an initial growth phase, cultivation temperature and pH were shifted.
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