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The intensity (Table 2) and appearance probability (Fig. 5) of 730 cm−1 increased significantly from 0 to 72 h, indicating that the DNA content in the biofilm was higher than that in the bacterial colony or initial attached cells.
In the present study, to fully understand the chemical variations during biofilm formation, SERS based on silver colloidal nanoparticles was applied to evaluate the chemical components in the matrix of biofilm at different growth phases, including initial attached bacteria, colonies, and mature biofilm.
The results can be applied for the analysis of various chemical components in the biofilm matrix of a specific growth phase (such as initial attached bacteria, bacterial colonies, or mature biofilm) and may provide new information related to the relationships between structures and functions during biofilm formation.
All of these indicate that, due to DNA accumulation or release, higher concentrations of eDNA might exist in the mature biofilm matrix than in earlier phases of biofilm (i.e., initial attached cells and bacterial colonies), as we observed in the present study.
Although the appearance probability of lipids was kept stable, the peak's intensity increased significantly for E. coli and P. putida from bacterial initial attachment to mature biofilm (Table 2), indicating that the lipid content in the mature biofilm was higher than that in initial attached cells or bacterial colonies.
Similar(55)
The initial self-equilibrated tensegrity structure had several initial boundary conditions as initially attaching on the substrate.
The spreading area was also normalized by the initial attaching area.
If it is shown that the first implies the second then the second inherits the initial plausibility attached to the first.
Initial coding attached basic surface-level labels to data, resulting in ∼600 codes.
In accordance with the protocol proposed by Begue et al. [ 10], the initial weight attached to the tail was 50% of the rat body weight and was increased progressively until 150% after 4 weeks (Table 1).
No defect is found in initial attachment, and once attached, protrusions extend rapidly.
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