Sentence examples for initial assembly with from inspiring English sources

We followed the initial assembly with several rounds of DQer, until no contig containing 15%% or more questionable (Q) clones.

We performed the initial assembly with tolerance of 5 (=0.5 bp) and Sulston score cutoff 1 × 10−65.

The very low fraction of Q clones shown that initial assembly with the cutoff value of 1e-60 was reliable.

There were 69 such loci longer than 1000 bp in our initial assembly, with the longest spanning 6501 bp.

We mapped these reads to the initial assembly with Bowtie2 [ 35] and calculated the coverage of each scaffold with the help of SAMtools [ 36].

There were 31 such pairs of loci longer than 1000 bp in our initial assembly, with the longest spanning 8946 bp.

A preferred assembly strategy is thus to perform initial assemblies with multiple high-quality assemblers and then to merge these using a traditional OLC assembler such as CAP3.

These observations, and the small number of rearrangements believed to distinguish the karyotypes of Saccharomyces sensu stricto yeasts (Fischer et al. 2000), prompted us to use sequence similarity to organize our initial assemblies with respect to the S. cerevisiae genome.

For all datasets, GeneStitch significantly improves the completeness of assembled genes as compared with initial assembly's genes (with higher gene coverage), and the number of complete genes, especially for the datasets with lower sequencing depths (6× or 13×).

The genome assembly of O. niloticus is in a more advanced state, contigs being organised into linkage groups or chromosomes, while the T. orientalis genome is in its initial assembly release with sequences organised into contigs.

To capture the 5' flanking sequence, we used human sequences and in particular a regulatory region (HSA15:74665162-74667983). We were able to propose an initial assembly (HQ687747) with four genomic fragments schematized on Figure 1.