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To measure the initial (and maximal) rate, enzyme assays are typically carried out while the reaction has progressed only a few percent towards total completion.
Kymograms were produced by extracting 1 pixel-width strips from each movie frame at points of initial and maximal lamellipodia extension, and assembled using ImageJ (v1.40).
Initial and maximal absorbance was 0.6.
Initial and maximal PCT levels correlated with maximum SOFA score (r2 = 0. 51 and r2 = 0.57, respectively).
To further test this hypothesis, we assayed both initial and maximal activities of NADP-dependent malate dehydrogenase and from these data calculated the activation state of the enzyme.
The amplitude of ICa was measured as the difference between the instantaneous current at the initial and maximal activating current values.
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Our results showed that the snout diameter at the start of the simulations is involved in a trade-off between the realizable suction volume and expansion time on the one hand (improving with larger initial diameters), and maximal flow velocity on the other hand (improving with smaller initial diameters).
V = frac{{V_{hbox{max} } times [S]}}{{K_{text{m}} + [S]}} frac{1}{V} = frac{{K_{text{m}} }}{{V_{hbox{max} } }} times frac{1}{[S]} + frac{1}{{V_{hbox{max} } }} where [S] is the concentration of the substrate, V and V max represent the initial and the maximal rate of the reaction, respectively, and K m is the Michaelis constant.
Initial claudicating distance and maximal walking distance were determined from the treadmill test.
After adjustment on IGS2, inappropriate initial antibiotic therapy and maximal dose of vasopressors remained independent prognostic factors, whereas V max E′ became quite not significantly associated with ICU death (p = 0.07).
After adjusting for the SAPS II score, inappropriate initial antibiotic therapy and maximal dose of vasopressors remained independent factors for ICU mortality, whereas a trend was only observed for lateral E′ maximal velocity (OR 1.11 [CI 95%% 0.99 1.23]: p = 0.07).
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