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Hybridization and initial analysis were performed by ATLAS Biolabs GmbH (Berlin, Germany) by using GeneChip Human Exon 1.0ST arrays (Affymetrix, Santa Clara, CA, USA).
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The initial analysis was performed with genes that were commonly DE among all samples.
An initial analysis was performed using a 34×2 contingency table and the null hypothesis stating that the number of complementation events is the same for each eIF4E mutant was rejected (P = 6×10−28).
An initial analysis was performed with MAS5.0 (Affymetrix) and Robust Multiarray Average (RMA) software [19], which indicated a very high variability that was associated with the biological variability and non-specific hybridization.
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