Sentence examples for initial amount of each from inspiring English sources

The qPCR data were converted to the initial amount of each target in the reaction (N0) using the LinReg software [68].

We then specified the initial amount of each element, the rate laws and the values of the parameters.

In each case, the kinetic constants were set to 1.0, the initial amount of each component is 100000 molecules and the simulation time corresponds to 5 time-units.

These values were divided to the initial amount of each biopolymer calculated from the total amount of dry biomass used in each experiment according to the composition reported in Table  7.

The primers used to amplify each gene are listed in Table 3. Thermal-cycling conditions included an initial sample incubation at 50°C for 2 min and at 95°C for 10 min, followed by 40 cycles at 95°C for 15 s and at 60°C for 1 min. The relative differences in the initial amounts of each cDNA species were determined by comparing their threshold cycle (CT) values.

The initial amount of cells in each flask was 2 g fresh weight.

This standard curve mean of y = −3.36x+23.72 (n = 8 experiments) derived from plotting the Ct values of each input amount (from 8 to 0.0008 cells) against log10 of the starting cell number, enabled us to calculate the initial amount of cells for each PCR sample.

When E 0) is equal to 12 (the initial amount of the substrate), each enzyme converts on average at most one substrate.

The initial amount of transcript in each sample was calculated from the standard curve using the default (fit point/arithmetic) method of LightCycler Software Version 3.5, and normalized to the values of actin or dap.

Expression of the gene of interest was normalized to house keeping genes (glyceraldehyde-3-phosphate dehydrogenase or GAPDH and β-actin) and the initial amount of the template of each sample was determined as relative expression versus one of the samples chosen as reference.

The results were normalized to 18S and β-actin, the initial amount of the template of each sample was determined as relative expression versus one of the samples chosen as reference (in this case the control sample) which is considered the 1x sample.