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Duplicate reads were removed after the initial alignment, to eliminate reads that mapped to the same position of the transcriptome.
As we considered only perfectly matching reads after the initial alignment to the genome, we could easily translate from color space to base space sequence format.
The pharmacophore alignment function of MOE was used to produce a rough initial alignment to ATP of the diverse input set based on common chemical features.
The alignments generated by Prospero were used as an initial alignment to make profile Hidden Markov models using HMMER [ 59], and the resulting alignments were scanned against the Swiss-Prot database.
We shifted the Illumina base quality scores (Phred+64) to the Sanger scale (Phred+33) [ 6] and performed an initial alignment to the National Center for Biotechnology Information human reference genome HG19 using the program Burrows-Wheeler Aligner (BWA) [ 7].
We shifted the Illumina base quality scores (Phred + 64) to the Sanger scale (Phred + 33) [ 15] and performed initial alignment to the NCBI human reference genome (version 36 for SureSelect data and 37 for TrueSeq data) using the program Burrows-Wheeler Aligner (BWA) [ 16].
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They search the neighborhood regions of the initial alignments to obtain locally optimal solutions, which improve the information content of the discovered profiles.
Common variations include the use of flexible alignment (Mosca et al., 2008; Rocha et al., 2009; Salem et al., 2010; Shatsky et al., 2004; Ye and Godzik, 2003) or using fragments and topological filters for initial alignments to improve quality and speed (Budowski-Tal et al., 2010; Gibrat et al., 1996; Krissinel and Henrick, 2004; Veeramalai et al., 2009).
This was a much better validation rate, as compared with just four of 28 (14%) of variants identified using a more traditional method, suggesting that D iamund has a higher specificity when compared with our traditional analysis, which is based on initial alignments to the reference genome.
In addition, subsequent alignment as the market develops, as opposed to initial alignment at the beginning of market emergence, is associated with the reconfiguration of capabilities and is the more important determinant of entry timing.
The core motif from the predicted promoters was further used to track the additional putative promoters that may be missed by the initial alignment due to shorter conserved motif length.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com