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Inhibitory activity was assayed by the addition of samples to a known amount of active collagenase in the diffuse fibril assay.
The DPP IV inhibitory activity was assayed according the previous report (Lacroix and Li-Chan [2013]) with modifications.
Moreover, the lipoxygenase (LOX) inhibitory activity was assayed following the method proposed by Tappel [ 43].
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SOD activity was assayed based on inhibitory effect of SOD on the spontaneous autoxidation of pyrogallol [ 50].
After the treatment, AMPK activity was assayed.
In our recent study, we designed several analogs of P-NT.II and their inhibitory activity was evaluated by in vitro inhibition assays against a purified human synovial sPLA2 enzyme.
The assay for HIV-1 RT inhibitory activity was assessed by using an enzyme-linked immunosorbent assay (ELISA) kit from Boehringer Mannheim (Germany).
In the current study, ACE inhibitory activity was determined using an ACE inhibitory assay kit (ACE kit-WST, Dojindo Laboratories, Kumamoto, Japan).
The anti-inflammatory effect was evaluated in carrageenan-induced rat paw edema model, while the lipoxygenase inhibitory activity was performed in the in-vitro assay.
The study of AChE inhibitory activity was carried out using the Ellman colorimetric assay with huperzine-A as the positive control.
p27 kinase inhibitory activity was also tested with an in vitro assay using recombinant cyclin-CDK complexes.
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