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As described previously [ 38], we found that co-expression of TBC1D20 inhibited traffic of VSVG EGFP (results not shown) and also effectively inhibited traffic to the plasma membrane of Kv4.2 which remained intracellular but still co-localized with KChIP1.
Treatment of Neuro2A cells with Vti1a and VAMP7 siRNAs specifically inhibited traffic of Kv4.2 channels to the plasma membrane and instead these remained, in part, in an intracellular compartment suggesting that the Kv4.2/KChIP1 traffic also involves Vti1a and VAMP7 in neuronal cells.
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The translational fusion of SUT1 to GFP may have inhibited trafficking.
Similarly, SUT1-GFP fusion protein was found in CC, suggesting that the fusion led to accumulation of SUT1 in CC and inhibited trafficking to SE. Accumulation of the fusion was confirmed by immunolocalization.
We plan to remove the asphalt plant from the waterfront altogether, and alternatively will pursue permit limits to inhibit traffic problems.
This model suggests that treatments which disrupt naive B cells such as BAFF inhibition may be particularly effective in the treatment of SLE and treatments that inhibit trafficking or retention in target tissues may be promising approaches for treating RA and pSS.
Conc A inhibits trafficking of vacuolar proteins and prevents vacuolar protein degradation [9], [49].
In addition, the expression of a KK-Mut Bves inhibits trafficking and/or accumulation of endogenous Bves at the cell surface (Figure 6K).
Since prior sound exposures that induce TTS (or greater pathologies) can increase hair cell uptake of gentamicin in vivo, interventions that inhibit trafficking of aminoglycosides into the cochlea likely represent one potential clinical strategy to prevent sound-then-drug ototoxic synergism.
Specifically, using dye transfer studies, disruption of CENP-F function inhibits trafficking of connexins, resulting in loss of cell-cell coupling (Pooley et al., 2008).
Expression of KChIP with single or triple EF hand mutations quantitatively inhibited Kv4/KChIP1 traffic to the cell surface but had no effect on VSVG traffic.
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