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Proteins were dephosphorylated with 3 μg of lambda phosphatase in the presence of 2 mM MnCl2, plus 10 mM EDTA for the inhibited control, for 30 min at 30°C.
Percent activity was computed after normalization against the uninhibited, or neutral, control (64 wells, entire columns 1 and 2) and the no-enzyme, or 100% inhibited, control (64 wells, entire columns 3 and 4), respectively.
Indeed, in some cases, the responses during this outbreak actively inhibited control and containment measures.
Accordingly, administration of the TβR-I inhibitor, SB-431542, inhibited control NMuMG cell wound closure, thereby identifying a role for autocrine TGF-β signaling in mediating the closure of MEC wounds.
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Under anaerobic conditions, generated with a N2 : CO2(g) headspace, two soils with nitrate or sulfate amendment showed an increase in extractable [14C]pyrene at 365 days relative to inhibited controls, presumably due to microbially mediated oxidation reduction potential and pH alteration of the soil environment.
In normal cells however, inhibition of CFTR significantly decreased Nrf-2 activity vs. non-inhibited control, by ∼80% in the 16HBEo− and ∼70% in the 9HTEo− cells, in the presence or absence of inflammatory stimulation.
However, when treated with a combination of glucose and pyruvate, ATP levels were restored to non-inhibited control levels.
However, a combination of glucose and pyruvate restored intracellular ATP levels to a similar level to that seen in non-inhibited control cells (Fig. 3F).
Verification of the adhesive nature of the PLGA mTG MPs was further investigated using the R281-inhibited control samples of the mTG (figure 5B).
However, the addition of a combination of glucose and pyruvate resulted in tyrosine phosphorylation levels comparable to the non-inhibited control.
The addition of either 5 mM glucose or 5 mM pyruvate to spermatozoa treated with NaCN did not recover the motility observed for the non-inhibited control cells (Fig. 4B E).
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