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The attacin E transgenic trees produced normal seeds, which enabled us to test the inheritance of transgene activity in progenies.
Fig. 5 Stable inheritance of transgene in BC1 orchid progeny.
The complex inheritance of transgene promoter methylation seems to suggest randomly incomplete erasure and reset.
We quantified this phenomenon in thousands of animals for each generation using flow cytometry and found that inheritance of transgene silencing was maintained in more than 60% of animals for at least four generations.
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Although there have been several reports on characterization of the integration pattern, expression and inheritance of transgenes in transgenic apple plants and their progeny [ 2, 15, 16], only a few studies have been published on the expression of transgenes during long-term evaluation in the field and the impact of the transgene on fruit characteristics.
The stable inheritance of transgenes of the transformed plants was evaluated by resistance to hygromycin in T1 generation.
Moreover, the inheritance of transgenes is stable in all the BC1 transformation lines tested in our genetic study as shown in Fig. 5.
Stable integration and inheritance of transgenes was demonstrated.
Chloroplast genetic engineering could minimize transgene escape because of maternal inheritance of transgenes [ 8- 11].
However, chloroplast genetic engineering offers the possibility of containment because of maternal inheritance of transgenes.
Two recent studies point out efficient control of maternal inheritance of transgenes in transplastomic tobacco.
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