To account for any bias inherent to the fluorescent dyes, a dye swap was performed such that within each treatment group (e.g. Medicated) half of the replicates were labelled with Cy3 and the other half were labelled with Cy5 at each time point.
Dye bias, an inherent problem to microarrays, identifies differential expression that is falsely attributed to the nature of the dye rather than to true differences in biological samples.
The F.D.A. scientists suggested that problems associated with artificial coloring might be akin to a peanut allergy, or "a unique intolerance to these substances and not to any inherent neurotoxic properties" of the dyes themselves.
However, it suffers from certain limitations such as those associated with the inherent property of the organic dyes that includes photobleaching, spectral overlapping, need for multiple excitation sources, and autofluorescence [21].
The above results present an efficient approach to improve the photovoltaic performance of the rutile-based solar cells by designing photoanode materials with hierarchical structures to counterbalance rutile's inherent shortages such as low dye-loading and poor conductivity as compared with anatase.
The inherent property of PANI to adsorb dyes has been explored for the development of electrochemical detection of dye in solution.
Having said so, natural dyes do have some inherent limitations such as poor tinctorial strength, lack of reproducibility, inferior fastness properties and un-exhausted metallic mordants in the residual dye bath posing serious challenges to the environment [3, 4, 5, 6, 7, 8].
This method can be used most efficiently with photo-switchable fluorophores, but it has been shown that dSTORM is also possible with conventional dyes, based on their inherent switching capacity [ 18].
This finding showed that PPy/MCM-41 pore structure changed with dye adsorption probably because of inherent disorder, and not the MCM-41 structure collapse.
Normalization was then performed using the q-spline method [ 86], compensating for inherent differences in signal between the two dyes.
In conjunction with Cy5 and Cy3, this dye has shown similar levels of inherent quality of labelling and subsequent data acquisition.
