Inhalation animal models are used to determine which components of a mixture are the most important toxicants for inducing the observed adverse outcome.
On the fifth and sixth inhalations, animals received a solution with 2.5 mg/mL of ovalbumin, and for the seventh inhalation, the dose was increased to 5.0 mg/mL.
Little is known about the precise effects of smoke inhalation on animals but the lungs of animals are similar to those of humans, so it is expected to make them sick and unable to feed.
The administration of aerosols for inhalation to animals, however, remains a critical challenge and only a few methods of administration have been developed.
Using heart puncture blood was collected in potassium EDTA tubes (Sarstedt, Nümbrecht, Germany) from Isofluran (Baxter, Kista, Sweden) inhalation anesthetised animals.
One hour after the last inhalation the animals were euthanized, and lung samples were removed.
Seventy-two hours after the 7th inhalation, the animals were anesthetized with thiopental (50 mg/Kg (ip), tracheostomized, and exsanguinated after thoracotomy.
Inhalation by animals or humans in the target site would be expected to result in rapid elimination in urine, resulting in a negligible hazard.
Approximately thirty min after the last inhalation, the animals were euthanized, a bronchoalveolar lavage (BAL) with 10 ml of 0.9% NaCl in each lung was performed, and lung samples were removed.
Berman et al. (1995) statistically analyzed the results of 13 separate animal inhalation studies, which exposed animals to nine different asbestos types.
