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Several ingredients were selected on the basis of AmB solubility and compatibility with skin.
For medium optimization, five ingredients were selected as variables, and among them yeast extract, tryptone, and sodium butyrate were selected as significant variables according to a fractional factorial experimental design, a steepest ascent experimental design, and a Box Behnken experimental design.
To further predict the targets and pathways, six representative ingredients were selected from the four herbs (Fig. 4a).
14 For their part, the anti-cellulite ingredients were selected on the basis of their potential complementarities in addressing the cellulite problem on all fronts, according to published literature.
The chemical constituents of CBPP were identified by high-quality ultra-performance liquid chromatography/quadrupole time-of-flight mass spectrometry (UPLC/Q-TOF-MS), and anti-inflammatory ingredients were selected and analyzed using the PharmMapper and Kyoto Encyclopedia of Genes and Genomes (KEGG) bioinformatics websites to predict the target proteins and related pathways, respectively.
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The ingredients are selected very early in the year, based on predictions of what strains will circulate the following winter.
Initial screening of the ingredients is done to understand the significance of their effect on the product formation and then a few better ingredients are selected for further optimization [8].
Non-aflatoxigenic active ingredients are selected from VCGs and/or SSR haplotypes that are relatively common and widely distributed in the target agroecosystem and which contain no aflatoxin-producing members (Atehnkeng et al. 2014; Bandyopadhyay and Cotty 2013; Cotty 2006; Probst et al. 2011).
Although the industry blocks attempts to know what chemicals and combinations are used, we know that it is a cocktail whose ingredients are selected from a possible menu of around 600 chemicals.
The ingredients, ratio and dose were selected based on their biophysical interrelationship with the total known multiple MS causative factors, their biochemical importance and the role they were expected to play in the normalisation and treatment of the involved complex network of events in the disease pathophysiology.
Seven ingredients used without strong abrasives were selected based on the combinatorial approach.
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