Direct flow infusion of the samples was performed on a Thermo Scientific LTQ XL linear ion trap analyzer equipped with an electrospray ionization (ESI) source, both in positive and negative modes (Thermo, San Jose, CA, USA).
The concentrations of essential and nonessential elements in tea infusion of the analysed samples were generally lower than the maximum values established by EU Drinking Water Directive 98/83/EC; thus, these tea can be considered safely for consumption.
In the previously published method direct infusion of immunoprecipitated sample to the MS was used [ 20] but we find that the infusion flow by itself has a major impact on the relative abundance of the spectral peaks (data not shown) (metal emitters have been reported to cause unwanted sample oxidation [ 33- 35]) and we here instead use reversed phase separation in line with the mass spectrometer.
We have previously demonstrated that intraseptal carbachol (12.5 125 ng) infusion immediately after the sample session of a delayed-non-match-to-sample radial maze paradigm produces a dose-dependent amnesia.
In the first setup, a continuous flow of sample and CL reagents was used, whereas in the second setup, a fixed volume (2 μL) of (Ru (bipy 32+) was introduced into a continuous infusion of peroxydisulphate and the sample.
For those patients receiving a continuous infusion of IFO, sample times were pretreatment, 3, 6, 12, 18, 24, 36, 48, 60 and 72 h during the infusion.
The mass spectrum for both methanol and aqueous fractions obtained by direct infusion of samples into the negative ion mode ESI-MS showed the presence of a major peak at m/ z 191 which was previously detected and identified in other Rosa species as quinic acid [ 21].
After a 10-h fast, subjects were admitted to the GCRC for the study, where two intravenous catheters were inserted into superficial veins for continuous infusion of the tracer solutions and blood sampling.
Different lines were used for drawing blood samples and infusion of the bacteria.
