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Different emitter setups were applied for continuous infusion, flow injection, and liquid chromatography/mass spectrometry experiments.
Performance in terms of antioxidant activity, reactivity of the oxidation product, and matrix effects (ion enhancement/suppression) is determined by continuous infusion, flow injection (FI), and liquid chromatography (LC)/MS experiments employing three different emitter systems for ionization to allow the selection of the most appropriate redox buffer.
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However, PC species were poorly ionized in negative ESI mode, most likely due to ion suppression from other phospholipids (PI, PE and PS) when direct infusion or flow injection was utilized.
This is one explanation of why a binary solvent mixture composed of methylene chloride and TFA – that is frequently applied in direct infusion and flow injection experiments – but also dried ACN, resulted in highest signals for radical ions.
Flow injection, continuous infusion, and on-line HPLC experiments were performed.
MS/MS settings were optimised on the Ultima PT and TSQ instruments using a direct infusion of individual standard solutions and flow injection analysis, respectively (Tölgyesi et al. 2012).
Analyte-specific multiple reaction monitoring conditions and ion source parameters were determined using an appropriate Analyst® feature, by the infusion of a 200 ng/mL solution of each carbohydrate, and in flow injection mode, respectively.
flow injection analysis system.
flow injection acquisition.
Batch and flow injection approaches were compared.
Flow analysis in general defines techniques for continuous flow analysis (CFA) and flow injection analysis (FIA).
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