Sentence examples for infrequent restriction from inspiring English sources

These contigs can be assembled at discrete, infrequent restriction enzyme cut sites, extending the sequence space used for SNP discovery and providing sufficient flanking sequence for high-throughput genotyping platforms such as the Illumina GoldenGate and Sequenom iPlex [11], [12], [13], [14].

Consequently, infrequent restriction site-PCR (IRS-PCR) methods and more recently ligation-mediated PCR (LM-PCR) were applied, taking into account this higher number of IS 711 elements, to study the genomic diversity of marine mammal strains [ 26- 28].

The cultured Enterobacter species were all identified as E. nimipressuralis through hsp60 gene sequencing and infrequent-restriction-site PCR (IRS-PCR).

Furthermore, we also performed infrequent-restriction-site PCR (IRS-PCR) [ 3] fingerprinting to confirm that the same bacterial strain had been isolated from the two patients and the salined cotton pad. Figure 2 shows that the three isolates yielded identical DNA banding patterns.

The spacer was designed to contain recognition sequences for infrequent cutting restriction enzymes near the extremities of the loop, as well as sites for efficient annealing of sequencing primers.

It is unclear if the BJ1 genome ever forms a circular molecule but if it does then cos sites are unlikely to be involved as digests with three infrequent cutting restriction enzymes (HindIII, EcoRV and EcoRI) followed by melting at 80°C failed to show any change in the number of bands compared to un-melted digests (data not shown).

Second, people in very good relationships may have infrequent contact due to restrictions, for example, because they live far away from each other.

An infographic created by private American healthcare group CRC that was circulating online described "almost anorexia" as affecting one in 20 women, and as involving frequent restriction, mild binge eating, infrequent purging and negative body image.

In this region of the plot, several inferior practices are grouped together: the combination of a lack of flow restrictions on trucks carrying replacement animals, with infrequent washing of trucks between loads and the lack of disinfectant use are particularly concerning.

Choice of restriction enzyme(s) (e.g. frequent or infrequent cutters) and the fragment size selection window can be used to optimize the number of tags, capturing a certain proportion of the genome for SNP detection and comparison across multiple individuals or populations.

Our aim was to experimentally compare the effects of one night of induced infrequent night-wakings (of ∼15 min, each requiring a purposeful response) and sleep restriction on sustained attention and mood in young adults.