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The broadband, infrared probe pulses are generated as synchrotron radiation in a bending magnet downstream of the OK-4 wiggler.
While the direct spatial structural information obtainable by in-situ STM makes this technique of obvious importance for exploring nanoscale phenomena, the infrared probe offers not only chemical specificity, but also the fast (∼10−11 s) spectral timescale enables fluxional adlayer structures to be probed.
Here, taking advantage of a constitutively active near infrared probe we have imaged in a living animal the neo-vascularization of early dysplastic lesions by micro-vessels, and revealed the change in architecture of the vasculature entering the lesion.
The relaxation of the WW domains was measured by both an infrared probe and a fluorescence probe.
In contrast, studies on sperm-whale myoglobin in D2O using a tuneable CW lead salt diode infrared probe laser, concluded that protein relaxation after photolysis of CO from Mb-CO is complete after 100 ns [ 8].
This result revealed that 1 can be used as an infrared probe for monitoring double stranded DNA.
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This can be monitored directly by near infrared probes or indirectly with temperature probes.
In summary, we have shown that live imaging of mechanism-based near infrared probes allow simultaneous detection of independent biological activities that report progressive tumor growth.
Altogether, these observations establish live imaging of cathepsin B activity with sensitive near infrared probes as a highly specific method for detection of biological activity linked with progressive tumor growth.
The data were normalized after being fit to an apparent two-state equilibrium model using eq 2. The melting curve probed by infrared is more broad than the melting curve probed by CD likely because infrared probes the secondary structure of the entire peptide whereas CD probes the chirality of the environment of the tryptophan residues.
The preparation and properties of lanthanide-coated nanoparticles are then discussed in detail as luminescent visible and near infrared probes.
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