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To be consistent with Peiffer and coworkers [4], we represent Ballele as the position of the upper informative line of focus, giving a range of 0.5 to 1.
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Therefore, the GECN information for wheat is important in selecting informative lines from an RH1 population or identifying mutants in an M2 population.
In 1966, Anne Sexton, who committed suicide eleven years after Plath, wrote a poem addressed to Plath entitled "Wanting to Die," in which these startlingly informative lines appear: But suicides have a special language.
A value of 1 means only one parental chromosome is present such that no SNPs are informative (lines of focus at 0 and 1) as is the case for complete LOH.
On average, each of the 70 informative lines carried 2.8 deletions, ranging from one to thirteen.
Increasing the number of markers for characterizing the RH1 lines also increased the proportion of informative lines.
Among the 399 informative lines, 15-28% of the lines had deletions for an individual D-genome chromosome.
It indicates that informative lines might have multiple deletions for all chromosomes requiring high density of markers for detection.
From this panel of 1,510 RH1lines, a subset of 178 most informative lines was selected for this study.
Three hundred ninety-nine lines (26.4%) in the AL8/78-DGRH1 AL8/78-DGRH1dentified using 35 SSR markers that contained at least one marker loss (hereafter called informative lines).
This indicates that more informative lines can be obtained from few crosses, thus, reducing the efforts required for developing a RH panel.
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