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For the identification of novel wheat miRNAs, we firstly rely on wheat EST sequences as miRNA surrounding sequences in prediction, since the information of wheat genome sequence is limited.
This advantage of the mRNA-seq approach is particularly valuable considering the limited current sequence information of wheat genes expressed during senescence and the lack of an assembled wheat genome.
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This data set is from CIMMYT global wheat breeding program and comprises phenotypic, genotypic, and pedigree information of 599 wheat lines.
Chromosome arm information is available for 423 and 313 genes located in chromosome 6HS and 6HL, respectively; therefore, we compared these barley genes with the arm information of our wheat assembles.
Further detailed analysis of the relevant genes should provide new information about mechanisms of wheat resistance to rust infection and supply novel candidates for modern plant breeding using molecular techniques.
Identification of the VRN-B1 allele in a global collection of tetraploid wheat could provide important molecular information for wheat breeding.
Our analysis could fail to identify the bona fide long npcRNAs in wheat due to the limited genomic information and gene annotation of wheat, however, these 125 putative long npcRNAs constituted a reliable set of wheat long npcRNAs.
This study can provide useful information for the improvement of wheat quality, as well as give a deeper understanding of the molecular and genetic processes controlling grain texture in bread wheat.
Puccinia striiformis f. sp. tritici causes severe yield losses in wheat, but the information of gene expressions in infected susceptible wheat cultivars is scanty.
This, taken together with the availability of a complete genome sequence and an increasing number of resources for functional genomics, gives Bd the potential to become a significant model species with which to investigate resistance to Fusarium species and provide information of direct relevance to wheat and other cereal crops.
The anchored contigs of chromosome 3B will provide the initial template for the sequencing of this chromosome generating an unprecedented amount of sequence information in wheat.
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