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We observed three classes of puncta: red, green and yellow (Fig. 5 and 6; Supporting Information Movie S1 and S2).
At 15 minutes the progression of the FRET signal can be seen in Supporting Information Movie S2.
Both heavy and light particles displayed heterogeneity of GFP fluorescence (Fig. 6; Supporting Information Movie S2), as has been observed for extracellular virions [35].
In contrast, mRFP puncta moving in the retrograde direction were generally not coupled with green fluorescence (122 out of 125 total) (Fig. 5 and 7; Supporting Information Movie S1).
We tested these strains for visual learning using a modified version of a walking paradigm that pairs visual stimuli (the conditioned stimuli) with shaking (the unconditioned stimulus, see Methods and Supporting Information Movie S1).
In contrast, reduction in size of a terminal colony resulted from the shrinking of each individual cell (Supporting Information Movie S2).
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Reference movies of birds' walking ability for each of the six categories are given in the supplementary information [Movie S1, Movie S2, Movie S3, Movie S4, Movie S5, Movie S6].
(DOC 166 KB) 11671_2011_586_MOESM2_ESM.ZIP Additional file 2 Supporting information: movies.
Content update: Provisioning of sporadic content updates for onboard servers for information and entertainment, e.g., annual time tables, advertising, news headlines, touristic information, movies etc.
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Figure 5 shows frames from time-lapse movies (Supplementary Information, movies S1 (control - MO) and S2 (CXCL12-MO)) which document the spontaneous pinching off from the plasma membrane of CXCR7 containing endosomes and their re-association with the membrane.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com