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Mass spectrometric analysis was performed by information dependant acquisition (IDA) using the Duospray™ source (SCIEX) with a 25 µm electrode at +5500 V (gas settings GS1 13; GS2 0; CUR 25; TEMP 0).
An Information Dependant Acquisition (IDA) experiment was used to specify the criteria for selecting each parent ion for fragmentation which included selection of ions in m/z range: >400 and <1600, of charge state of +2 to +5, exclusion of former target ions for 30 seconds, accumulation time of 1 second for a full scan and 2 seconds for MS/MS, ion source voltage of 2200 V and temperature 120°C.
For information dependant acquisition, the MS survey scans were acquired in 250 ms and the top 30 product ion were selected for MS/MS scans.
MS data acquisition was performed using Analyst 1.4.2 software (Applied Biosystems) in the positive ion mode for information dependant acquisition (IDA) analysis.
For enhanced product ion scan the information dependant acquisition (IDA) criteria was as follows: select 1 - 2 most intense peaks which exceeds 10,000 cps, exclude targeted ions after 3 occurrences for 12 s.
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Moreover, this exchange of information was dependant on oral communication, as clinical records were never written concurrently.
More than 2 years of SWARM data together with monthly means from 160 observatories available till March 2016 were used to update the CHAOS time-dependent model to provide information on the time-dependant behaviour of the core main field part of the geomagnetic field between 1999.0 and 2016.5.
Distributions of 4-day rainfall prior to days with freezing events provide quantitative information on the geographically dependant frost decay risk in porous, mineral building materials in a given climate.
Second, information on independent and dependant variables were collected using self-reports.
Management strategies for coral reefs are dependant on information about the spatial population structure and connectivity of reef organisms.
To obtain predictions for the therapeutic response of individual patients, one needs to interpret spatially dependant transport information from DCE-MRI.
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