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As shown in Figure 4 and Figure S6 in the Supporting Information, cleavage of the His-His bond resulted in ions m/ z 1511 and m/ z 1773, indicating that the oxidized His residue is on the long chain highlighted in red.
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Accordingly, we developed a two-phase mapping pipeline that uses cleavage information to extract from these chimeras informative contacts that involve two, three or four loci.
The probe was employed as DNAzyme substrate to convert directly the cleavage information into fluorescence signal in real time.
However, little information concerning optimal cleavage site motifs for Drosophila caspases is available for useful comparisons to be made.
Based on theoretical estimates (supplemental information S1), the cleavage kinetics of the probe are faster than cellular uptake by activated macrophages, so the enzyme kinetics were ignored.
This experiment yields single nucleotide resolution cleavage information for the entire 540 nucleotide bI3 splicing precursor in one high-throughput experiment (top panel, Figure 1A).
After mapping, the information of exact cleavage and polyadenylation sites was extracted.
The primary source of protein cleavage information is the published literature.
However, the cleavage information from isocitrate-ICL complex structure is needed, which is unavailable at the moment to further confirm the abovementioned hypothesis.
In miRGate, we stored human, human viruses, mouse and rat miRNA sequences (Table 2), as well as other available information such as cleavage data from pre-miRNAs to mature miRNAs, genomic coordinates and family names.
Because MALDI-TOF/TOF systems display higher glycan ionization efficiencies than electrospray ionization MS systems, they might potentially reveal cross-ring cleavage information of N-glycans when using high-energy collision-induced dissociation [ 34– 34].
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