Exact(3)
"By channeling Celera's unique genomic information through Applied Biosystems' worldwide sales and marketing organization and combining it with other AB products, we expect to provide customers with unprecedented integration of information, assays, products and services," said Applera's White in a prepared statement.
In 91% of cases with receptor status information, assays were performed using immunohistochemistry, with biochemical methods used in the remainder.
We verified that diversified populations had higher frequencies of resistant mutants than the nondiversified populations (see Supporting information, Assays 1 and 2 in Data S1; Fig. S1).
Similar(57)
And third, we verified that streptomycin was still present in sufficient concentrations to kill susceptible cells after 72 h of incubation with bacteria (see Supporting information, Assay 4 in Data S1).
First, we estimated the spontaneous (i.e. without antibiotic pressure) mutation rate from susceptibility to streptomycin resistance to be c. 1 cell in every 3.94 × 10 (see Supporting information, Assay 2 in Data S1).
Second, for an initial single resistant cell to attain a carrying capacity of c. 2 × 10 cells, 31 doublings would be necessary, and based on our estimate of a maximum rate of c. 40 min per doubling (see Supporting information, Assay 5 in Data S1), this would require about 21 h.
At 24 h postinoculation, 20 μL of each population was plated on KB-agar for subsequent counting of colony forming units (CFUs), and streptomycin was added to each vial at 0, 50, 100, or 200 μg/mL (for estimation of MIC50, see Supporting information, Assay 3 in Data S1).
The third one is the "assay", which provides information on assays from which the functions are suggested.
GLIDA does not explicitly provide literature information for assays or tested substances in them.
(Additional information on assays, procedure and statistical analyses is available on request to the corresponding author).
The CPPs combined information within assays and cell lines using a robust two-stage hierarchical model of toxicity.
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