5) Please include information describing RDR2 expression and purification and remove the "data not shown" parts of the Results and Discussion.
Since there is no information about the isolation and purification of ARG from camel liver, this investigation was designed to purify and characterize ARG from camel liver and compare its molecular and kinetic properties with that reported from other species.
Detailed information about primer sequences, PCR conditions and purification methods are available in supplementary information (Additional file 1: Table S2).
In the ESNOQ assay it is important to preserve accurate S-nitrosation quantitative information after the biotin-switch and purification steps.
See the supplementary information online for materials, expression and purification of proteins, substrate preparation, the details of the implementation of QCM-D, ellipsometry, AFM indentation assays and imaging.
However, as shown in Figure 10b and Supporting Information, Table S3, after enrichment and purification using the MHM affinity microspheres, many peptides could be detected with strong intensity and clean background.
Some of the major disadvantages of HTL include the high operating pressure associated with the process (can lead to high capital investments and operational issues), lack of information on standard product separation and purification, and insufficient knowledge about large-scale process development.
Further information on chemicals, analytics, description of the syntheses and purification protocols are given in the supporting information.
For implementing an expert system, it is necessary to divide the separation process in two parts: recovery and purification, because the information and available heuristic knowledge are different in each part.
Analytical data and details of the syntheses and purification are described in the Supporting Information.
For more information about the various techniques for isolation and purification, see separation and purification.
