However, we find that proteasome inhibitors significantly increase surface STIM1 levels and peak Ca2+ influx upon TG-induced SOCE.
We next analyzed the effect of incubation of DCs with these antibodies on calcium influx upon BCG stimulation.
As shown in Figure S4, inhibiting PLCγ completely blocked increase in calcium influx upon blocking VGCC (panels b and c).
Blocking L-type (red line) or R-type (green line) VGCC in CFP10-DCs induced a significant increase in calcium influx upon M. tuberculosis WCL stimulation.
On the other hand, a HaCaT cell line established to express TRPV1 ectopically and primary DRG culture readily showed 45Ca2+ influx upon challenge with these agonists.
To this end, we suspended L-type and R-type VGCC-blocked-CFP10-DCs in calcium sufficient (culture medium) or calcium deficient medium (PBS) and measured calcium influx upon BCG stimulation.
As previously reported [18], STIM2 has lower ER Ca2+ sensitivity than STIM1 and therefore STIM2 translocates to puncta with ORAI1 and activates constitutive calcium influx upon smaller decreases in ER Ca2+ concentration compared with STIM1.
(A ) mPiezo1 mediates Ca2+ influx upon mechanical activation.
