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Therefore, the influx flow rate of each section is a function of the location and is defined by the well flowing pressure at the node.
Anklam (2005) proved that pressure in the well increases along the pipe moving from the hill to the toe region, and the influx flow rate decreases in the same direction.
The cell density of each culture was determined by counting DAPI-stained cells using a Cytopeia Influx flow cytometer.
Cells or no-cell controls were sorted directly into PCR-compatible microtiter plates using an Influx flow cytometer (Cytopeia, BD).
After trypsinization, cell pellets were resuspended in DAPI solution (2 mM CaCl2, 22 mM MgCl2 0.1 mg/mL BSA, 0.1% Nonidet P-40, 10 µg/mL DAPI, 10% DMSO) and run on an InFlux flow cytometer (Cytopeia).
Stained cells were analyzed on a Becton-Dickinson Influx flow cytometer.
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The electric influx flowing through the wires is directly controlled by the electronic music, so the artist's face becomes the visual element of the show.
The morphological changes were measured by BD-influx flow cytometer (BD Biosciences, USA) according to cell sizes and granularity defined in the forward light scatter (FSC) and side light scatter (SSC) plot.
Cells were then stained with 10 μg/ml Propidium Iodide (Sigma-Aldrich, P4170) for DNA content analysis by use of a BD Influx™ Flow Cytometer (BD Biosciences).
For analysis of Ca2+ influx by flow cytometry, cells were loaded with Fluo-4 (Life Technologies) for 30 min at 37°C in PBS containing 0.05% BSA, washed once, and resuspended in the same buffer containing 2 mM CaCl2.
Compounds were evaluated by fluorescence-based thallium influx assay, Rb+ flow assay and electrophysiological patch-clamp assay.
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