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Nasal and throat swabs were collected from each FARI episode for influenza detection by real-time reverse transcription polymerase chain reaction.
Currently accepted gold standard methods of influenza detection, viral culture and rRT-PCR, are time consuming, expensive and require special training and laboratory facilities.
An isothermal nucleic acid amplification test that offers the potential for rapid molecular testing at the clinical point-of-care with simple equipment can improve influenza detection rates.
The usefulness of this design procedure is demonstrated by applying it to a column-based NA purification device for influenza detection for a case study of Madin Darby canine kidney cells infected with influenza A virus.
PCR, another method used for influenza detection, is more specific, more sensitive, and less time-consuming, compared with traditional methods [12].
We looked at all data that we had regarding influenza detection since January up to this month and also to compare to the past season of influenza.
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No influenza detections were found in our study.
Influenza hospitalizations (KPSC and county-funded facilities) and peaks in influenza detections in Figure 1 show the relationship between EMS diversions and influenza activity.
We have previously shown that, although the syndrome of ILI corresponds to influenza detections in our laboratory [ 13], only about 40% of all ILI diagnoses by sentinel general practitioners in Victoria between 2003-7 were confirmed as influenza [ 2].
Bacterial culture is the gold standard for H. influenzae detection.
H. influenzae detection was done using primers that target the Outer membrane lipoprotein P2 (ompP2f, ompP2f:5'-GGTGCATTCGCAGCTTCAG-3' and ompP2r:5'GATTGCGTAATGCACCGTGTT-3'[ 25].
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