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Primary (all variables) and secondary (all influential markers, VIP > 1.0) prediction models were generated by the PLS method (Table 1).
The influential markers behaved in the order of plaque bacteria > diet > saliva, with previously known plaque bacteria/diet markers and a set of new protective diet markers.
Secondary models were also generated from blocks (bacteria - diet - saliva) or sets (single - five - multimarkers) of influential markers for comparative purposes (Table 1).
The complex variable patterning for baseline caries was highly reminiscent of those for increment and incidence, while that for progression was less complex with plaque bacteria as the main influential markers (Table 2).
These encouraging results, on a limited set of poor quality samples, could also be related to the classification method used, as the GGA-based class prediction strategy aimed at finding the most influential markers for each AML class (top markers with the highest inter-class variance and the lowest intra-class variance).
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Not all potentially influential inflammatory markers were measured.
The few influential saliva markers, for example calcium and phosphate, are consistent with current re- and demineralization models, but should - as other individual saliva or diet markers - not be over emphasized due to the multiple comparisons.
Fortunately, in this regard, Venice's opening slot has in recent years become a powerful and influential industry marker.
The secondary models harbor influential (VIP > 1.0) markers from the corresponding primary models M1 to M4.
The entire set of plaque, diet and saliva variables (n = 88) displayed a wide but shallow gliding scale of one fifth caries promoting or protecting markers and four fifths non-influential variables (Fig 3).
Furthermore, we cannot be completely certain that rs9340799 is more influential than rs2234693 because of the strong LD between these markers, separated by only 46 bp.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com