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This chemical functionalization influences the affinity of the formed pentadecane grafted GO sheets for various solvents.
We have studied how the modification of the RNA aptamer evolved against neomycin B at 2′ position of ribose with a methyl group influences the affinity of the interaction.
Together, these results suggest that the microenvironment of the peptide ligand influences the affinity of the integrin peptide interaction and that weaker interactions display a density-dependent enhancement of binding during cell attachment and spreading.
Presumably, the matrix (pH, ionic strength, and so forth) influences the affinity of folates for the binder.
Alternatively, allosteric ligands can change the receptor conformation in a manner that influences the affinity of ligands for the orthosteric sites and/or that change the coupling efficiency of the receptor to downstream signalling pathways.
Although we had no evidence to prove that rs2607420, the intronic SNP, directly affects the splicing efficiency or influences the affinity of transcription factor binding site, however, another intronic SNP of ITPKC, rs28493229, had demonstrated that the variant of rs28493229 is associated with the mRNA expression level of ITPKC [ 11].
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Furthermore, PTM sites are often located within short peptide motifs, modification of which influences the affinity for interaction with diverse binding partners (see section 3.1).
It is also likely that the stability of organo-Cu complex influenced the affinity of Cu towards humic organic matter (Wang and Qin 2007; Morillo et al. 2004).
The dissociation constant Kd of the AChE/AFB1 HRP interaction (0.4 μM) obtained with the surface plasmon resonance (SPR) technique is very close to the inhibition constant reported in amperometric assay (Ki = 0.35 μM), proving that the conjugation of AFB1 to a carrier protein does not significantly influence the affinity of AFB1 for AChE.
Also, the presence of the detergent DM could influence the affinity of the interaction.
We therefore examined if ARHGEF7 can influence the affinity of LRRK2 to GTP-sepharose.
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