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The influence sequences of the parameters on the properties of plasma-sprayed Al2O3-40 wt.%TiO2 coating are: spraying distance, spraying voltage, spraying current, argon gas flow rate.
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The influence sequence of factors is: temperature > strain rate > w/c ratio > maximum aggregate size.
Upon generating 55 sequences, the results imply that these two factors do influence sequence representation, and that the typical or representative sequence is one dominated by lakes.
Additional file 1: Figure S4 clearly shows that the influence sequence of Re3+ was as follows: Eu3 + ≈ Sm3 + > Y3 + > Pr3 + ≈ La3 + > Tb3 +.
Sample 4 prepared with slightly higher PEG concentration showed no difference in average read length indicating that PEG precipitation concentrations within 0.6% does not significantly influence sequencing results.
Figure S1: Reference mapping biases influence sequence alignment.
In addition, the read-lengths generated during sequencing influence sequence assembly, gene prediction and subsequently the functional analysis.
AA-ALIGNER allows key parameters to be specified that influence sequence alignment and post-alignment steps, such as imbalance detection.
Since cytosine methylation is associated with elevated accumulation of CpG to TpG transitions, epigenetic events might influence sequence conservation and result in unusual CpG contents of DNA sequences.
It has been reported previously that the gender in which a gene is expressed often acts to influence sequence level variation within and between species [ 55].
These genomes were chosen as representative bacterial genomes because they have considerably different GC contents and genome complexities, both properties known to influence sequencing quality and assembly processes.
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