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Two truncated recombinant CCHFV NP antigens were prepared based on results of prediction studies to include epitopic regions and exclude hydrophobic regions that could influence protein expression and solubility.
Moreover, the truncation position of the transit peptide from the N-terminus of CrGES was found to influence protein expression and activity significantly, obtaining a titer of 191.61 mg/L geraniol in strain with CrGES truncated at S43 (t3CrGES43
Alternately, dopaminergic activation may provide a mechanism by which environmental stimuli that alter dopaminergic activity may influence protein expression and/or sexual differentiation of brain and behavior.
MicroRNAs can influence protein expression by changing either translation from existing mRNAs, or by influencing mRNA stability and hence transcript abundance.
They influence protein expression by integrating into an active RISC complex capable to bind at a specific site to the target messenger RNA (mRNA) [3] thereupon inducing either cleavage of the identified target mRNA or stopping the translational process [3].
Second, it may be hypothesized that the genomic structure of the SLC10A2 gene with the G allele is the major determinant of the diminished ASBT expression though post-transcriptional mechanisms could influence protein expression.
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These findings led the authors to conclude that chronic NaHCO3 ingestion was influencing protein expression associated with slow but not fast-twitch fibres.
Although successfully used for heterologous gene expression for more than twenty years, general knowledge about all factors influencing protein expression by Pichia pastoris is still lacking.
It has been shown earlier that p53 can directly bind to the promoter regions of Oct4 and Nanog, thus influencing protein expression levels [31].
In animals, miRNA is thought to have a repressive effect that influences protein expression, not the mRNA levels.
Thus translation rate and the half-life of mRNA molecules will be affected, in this way influencing protein expression levels, and even altering the spatial structure of protein.
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