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In addition, it is possible that the fifth helix of PFN1 gets elongated under the influence of this mutation and the consequences of this are discussed below.
Once a mutant version of the bait (A) is found, one can study the influence of this mutation on the phenotype.
To check the influence of this mutation on the protein structure, both structures were subjected to energy minimization using AmberFF99 force field as implemented in SYBYLx2.0, Tripos Inc., USA.
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Regarding a potential influence of the mutation type on prognosis, our results, in contrast to the RASCAL study, show no differences with respect to mutation type.
The paternal influence of the mutation on the muscle phenotype indicates that a paternally expressed gene is likely to be causal.
There is also a strong influence of the mutation rate at which populations evolved towards the previous stationary state in their ability to adapt to new selective pressures.
The influence of the mutation rate on the degree of adaptation attained at the stationary state, and on the genotypic and phenotypic diversity of the population are questions that have been addressed previously with this model [9], [42].
We prefer the second choice, as only a higher ∆∆ G threshold can reflect the direct influence of the mutation.
The precise influence of this and other mutations of the NF1 gene on the resulting phenotypic expression remains to be elucidated.
Pearson's product moment correlation (r) between the number of transitions and the number of amino acid substitutions was 0.004 (F test, P = 0.972), which reveals the null influence of this type of mutations on the diversification of the two proteins.
Of the patients with V180I gPrD, 78 cases were of male patients and 108 cases were female, indicating a possible gender influence on the susceptibility of this mutation in the disease.
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