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Figure 3B shows the large number of EGFP+ cells infiltrating the eye, a site of major embryonic macrophage infiltration detected with the CSF1R-EGFP transgene previously [ 21], and also observed in mouse Csf1r-EGFP transgenics [ 12].
The interface at eroded dentin appeared very prone to degradation as was shown by the increased amount of adhesive failures and by the silver infiltration detected by TEM.
The infiltration detected in the Vps15-depleted muscles consisted of immune cells, macrophages and T-cells, as revealed by immunostaining with the F4/80 and CD3 markers, respectively (Supporting Information Fig. 7A).
Further, wild type EWAT exhibited higher frequency of larger adipocytes, indicative of adipocyte hypertrophy, which is associated with a decrease in adiponectin secretion (Table 1) and increased local inflammation with macrophage infiltration, detected as crown-like structures (CLS).
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Monoclonal conjugated antibodies for CD3, CD4 or CD8 were used to detect infiltrated T cells and monocyte infiltration was detected with the conjugated monocyte/macrophage marker mac-1.
Two distinct patterns of infiltration were detected, that is, diffuse periglandular infiltrates and leucocytes clustered into follicle-like aggregates.
MOG-EAE and Aβ42-EAE caused large clusters of infiltrated T cells, whereas no T-cell infiltration was detected in the coimmunized animals, thus resembling the situation in naïve mice.
Indeed, in the B16 tumours, no major change in the immune system infiltration was detected, while in the LPB tumours, after ECT with 10 μg BLM, a massive infiltration of immune system cells was observed at the time points where a large fraction of apoptotic cells was also detectable (25 100 h after the treatment).
No Ly-6G-positive cell infiltration was detected in the sham group of wild-type and IL-17A knockout mice (Fig. 5).
No infiltration was detected on retinal sections (left panel) of untreated eyes.
In contrast, neither age-matched AID+/+ (Figure 1A) nor AID+/− mice (data not shown) developed TLOs or gastritis, although minimal lymphocyte infiltration was detected in the gastric tissues of occasional mice in these control groups (Figure S2, and data not shown).
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com