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Genes encoding the inferred sequence and its N-terminal ATPase domain were PCR constructed and expressed in Escherichia coli.
Such a filtering strategy places the potential somatic mutation(s) in the contaminated cell into the inferred sequence of the contaminant and cannot distinguish the shared heteroplasmic mutations between the contaminant and the cell.
The inferred sequence (WAI/LKPFNR) of a peptide (m/z 1031.58), which is detectable in all trypin-digested extracts of protein spots 1 and 5, shares homology with the structural sequences from phages and viruses, such as mimivirus (Table S2), but does not match with host abalone hemocyanin subunit sequences.
In our GraphViz visualization, each circle represents a unique inferred sequence.
Sequence identifier, GenBank accession number and inferred sequence origin are given.
The inferred sequence for each species was aligned with the A. gambiae sequences and used for subsequent analyses.
Similar(47)
The lineage-specific Ka/Ks ratios were computed by the comparison between the inferred sequences at the human mouse ancestral node and the sequences at the human or mouse node.
These parental sequences (T0-66 and T0-55) were involved in other recombination events detected in the T0 sample and the T2 resulting daughters (except T2-52, only detected in the joint analysis), T2-58 and T2-76 were detected in the T2 sample too, but parental inferred sequences for these events had lower similarity or were unknown (see Figures 3 and 4).
Most of the HRM phenotypes that did not match the inferred sequence-based genotype were assigned as putative homozygotes from sequencing but observed as heterozygotes with HRM (data not shown).
We used the inferred sequences for P. vampyrus IFNA, IFNB, IFND, and IFNK to design oligonucleotide primers for cloning and sequencing and recovered a total of 110 sequences.
We used the inferred sequences to design sequencing primers and make several clones from each of IFNA, IFNB, IFND, and IFNK.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com