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This inferred Group III "seed plant duplication" genes would be congruent with that in its sister clade (which produced Groups I and II), increasing the likelihood of a WGD influencing both events.
This view is reinforced by inferred group membership, which seldom places an individual with high confidence (>90%) in one of the groups.
The percentages of membership (q), or inferred group ancestries, of the individuals in each of the two clusters were computed.
Individuals from the NE (Russia, Belarus + Estonia) populations clustered as only one inferred group with an assignment probability (Q) higher than 0.93 (Fig. 4b).
Mean values would likely have been higher, but populations adjacent to the crest of the Cascade Mountains (demarcating range limits of coastal and inland types) appeared admixed between lineages to varying degrees, substantially lowering inferred group fidelity.
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However, the inferred grouping was consistent across several analyses and with previous phylogenetic results (Seibold et al. 2011; Galgóczy et al. 2013a; Garrigues et al. 2016).
10,000 bootstrap replicates were run to estimate reliability of the inferred groups.
Average values of FST for the inferred groups were calculated through the program Powermarker.
The geographical origin of the accessions and their membership to the inferred groups are represented in Figure 3.
The inferred groups between successive K values were decided to identify the real number of clusters of individuals based on Evanno's methods [ 54].
The AMOVA for the thematic collection showed that 8.7% of the variation was caused by differences between the two inferred groups.
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