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Prediction of gene function, performed as described in the Methods, provided inferred functionality for 1,229 of these protein-coding genes.
We can make several predictions based on the inferred functionality distributions.
In all three cell lines examined, the three CTCF motifs exhibited pronounced differences in inferred functionality.
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The binding interactions of sVEGFR1, in particular, were inferred functionalities based on the first six Ig-like domains that it has in common with VEGFR1 (Fig. 1D).
Although Gene Ontology has its limitations as it is biased towards what is already known, it is still a universal key tool for functional annotation inferring functionality based on sequence identity, domains and structure, and literature studies.
In this paper, we used evidence of natural selection to infer functionality of an intronic region implicated in prostate cancer [7].
For example, Thomas et al. (2003) specifically use conserved regions of coding sequence to infer functionality.
Yet, since the joint contribution of multiple 'doses' is likely to determine transcriptional outcomes, it may be difficult to infer functionality of a given TFBS in isolation from others.
First, the short lengths of the identified ORFs and their general lack of detectable homology or reading-frame conservation with related species make it difficult to infer functionality [ 37, 38].
Detailed mouse, fly, and yeast GO annotations therefore, respectively, facilitate inferred putative functionality of orthologs across the vertebrate, arthropod, and fungal lineages.
Although this does not directly infer functionality, the majority of these twenty genes has been lost in the obligate endosymbionts of the mealybug, and some of these nuclear encoded genes complement the genes retained in the endosymbionts leading to complete pathways.
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