Exact(3)
High quality video imaging technology now makes it possible to observe and record the biology of chlamydial infection in detail Technical difficulties of measuring infectivity present problems of standardisation because some strains of C.trachomatis have high particle to infectivity ratios and need to be centrifuged onto host cells to initiate infection [8].
Preparations of gH-negative, gD-negative and gB negative virions had particle to infectivity ratios of 2×1010, 5×106 and 3×106 respectively.
In particular, in inherited prion disease, PrPSc isoforms may be generated with unique physicochemical properties, reflected by sensitivity to PK digestion and PrPSc/prion infectivity ratios that can be very different from the PrPSc types propagated in sporadic and acquired forms of human prion disease (for reviews see [ 2, 3, 22]).
Similar(56)
Preparations of WT virions had a particle to infectivity ratio of 3×101.
The average cattle∶sheep infectivity ratio is also very close to the values estimated using the original data.
For comparison, the infectivity ratio of wild type SV40 is 1∶200 to 1∶500 ([48] and our unpublished results).
To avoid this we have used the standard LGV strain L2 which has a particle to infectivity ratio of 1.0 and does not require centrifugation.
This is a within species ratio, a parameter directly fitted by the models, unlike the between species infectivity ratio additionally calculated as explained later in the text (see Parameter estimates section).
Rather than mentioning animals' specific infectivity (see Figure 3d and Table 1), it is more informative to comment on the cattle∶sheep infectivity ratio parameter for the most complex fit (this ratio does dot appear in the tables as it is not a model parameter).
Studies have shown that the infectivity ratio is directly related with the number of cysts inoculated to the experimental host [ 19, 20].
19 21 3T3-L1 cells were also infected with Escherichia coli previously isolated from the mesenteric fat biopsy specimens of a CD patient or with Lactobacillus spp. (infectivity ratio: 100 bacteria/cell) for 90 min.
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