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These results are consistent with the previous demonstration that infectivity is present in the liver from BSE-infected sheep 16 months after challenge as shown by mouse bioassay [7].
Since recent studies could demonstrate that scrapie infectivity is present in various tissues and body fluids of infected animals [38], [39], it has been assumed that the persistent prion protein enters the environment by contaminated excrements, birth-related tissues such as placenta, or even whole carcasses.
Data from blood transmission studies in sheep suggest that blood infectivity is present early in the incubation period, whatever the primary route of infection.
On the basis of these findings, we estimated that if prion infectivity is present at all in sCJDMM1 urine, it is at most 0.38 IU/mL if the 20-fold infectivity loss is factored in.
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It is inevitable that should infectivity be present in the milk of BSE-infected sheep, the risk to humans would be particularly dependent on the prevalence of BSE infection in sheep in the national flock.
These results indicate that 1) squirrel monkeys are susceptible to infection with the BSE agent, and 2) BSE infectivity was present in the bacteria-free filtrate used to expose cell substrates.
After 90 min digestion, ∼20% of the starting infectivity was present in both samples.
The present study used a short-observation transfusion model in the natural host to determine if relatively high infectivity was present in the total PBMC population, a CD72+ pan B lymphocyte population, a CD21+ subpopulation of B lymphocytes and either platelet-rich or platelet-poor plasma isolated from the blood of classical scrapie-infected sheep.
The incubation times recorded in this study would suggest moderate but significant levels of infectivity are present in the spleen of the MV genotype recipient, raising the possibility that other peripheral tissues and the blood of this individual are also infected, as indicated by immunohistochemistry in the initial report of this case (Peden et al., 2004).
These data show that 15 days after inoculation only about one LD50 of scrapie-infectivity is present in the spleen of turbot, and much less in the brain of turbot or in the spleen of trout.
Patterns of VL stratified according to markers of infectivity are presented in Figure 2.
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