Sentence examples for infectious yields from inspiring English sources

By also transfecting in vitro-transcribed M3 plus-strand RNA containing silent mutations that render it resistant to the small interfering RNA, we were able to complement μNS expression and to rescue infectious yields by ~ 100-fold.

In the current study, small interfering RNA targeting M3 was transfected in association with MRV infection and shown to cause a substantial reduction in μNS expression as well as, among other effects, a reduction in infectious yields by as much as 4 log10 values.

Infectious yields were assessed by plaque formation assay.

A serial dilution of the specimen cultured onto coverslips in 24 well plates with 20 hr growth 2 × 10 HEp-2 cells to determine the viable infectious yield [ 46].

To this end, cell culture supernatants were harvested to determine the infectious virus yields (infectious progeny production/release) while the MDCK cell monolayers were inspected for the presence of infected cells forming foci (clusters of infected cells) using immunofluorescent detection of the viral NP antigen.

To analyze whether viral DNA replication was altered, viral genome replication was quantified at 24, 28, 32 and 36 hours post infection (hpi) and correlated with infectious virus yields at the same time points.

At each time point, the infectious virus yields (TCID50/mL) were determined by titrating the supernatants on MDCK-SIAT1 cells (17 ).

The infectious virus yields of WT and the oseltamivir-resistant virus were reduced by >2 logs at 1 µg/mL of amantadine (data not shown), which is consistent with the M2 blocker sensitive genotype.

To identify functional roles for these core SUMO targets during IAV infection, we depleted A549 cells of the corresponding 42 genes one by one using small hairpin RNA (shRNA -expressing lentivirushRNA -expressingne), and we determined the subsequent replentivirusesIAV by measuring infecthree virus yields at 24 and 48 hr pert-infection.

We classified a host gene as important for IAV replication if at least two of three shRNAs increased or decreased infectious IAV yields by at least 5-fold at a minimum of one time point compared to the non-targeting shRNA.

Next, the antiviral activity, either based on quantifying the effect of the compound on the infectious virus-yield and/or the effect on viral RNA production, is confirmed in virus-yield assays.