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The infectious endpoint titers of HCVcc were determined from production of cytopathic effect after infection of Huh7.5.1 cells with tenfold dilutions of virus-containing cell-free supernatant in duplicate.
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We compared these registries for infectious endpoints after primary THA or HA over the years 2005 2009.
The two trials were aggregated: although infectious endpoints were identical, their metabolic endpoints differed slightly (Table 1).
Excellent lower limits of detection relative to viral culture were observed for the PCR assay by using 38 of 40 rhinovirus reference strains representing different serotypes, which could reproducibly detect rhinovirus serotype 2 in viral transport medium containing 10 to 10,000 TCID50 (50% tissue culture infectious dose endpoint) units/ml of the virus.
The tissue culture infectious dose 50% endpoint titers were calculated by the method of Reed and Muench and are reported in log10TCID50 per milliliter [40].
Viral titers from tissue culture supernatants were determined by titration on Vero cells and expressed as tissue culture infectious dose 50 percent endpoint titers (Log10TCID50/ml) as previously described [63].
Plates were incubated at 37°C; CPE was monitored; and 50% tissue culture infectious dose per milliliter endpoints were calculated.
Few studies have demonstrated the capacity of enteral nutrition to reduce infectious complications, improve nutritional endpoints, or decrease mortality [ 1, 23].
Final purified viral stocks were titered by determining their concentration (particles/ml) by optical density at 260 nm (1 OD260 unit = 1×1012 particles/ml), and their infectivity (infectious units/ml) was measured by endpoint dilution assay.
The primary endpoint was an infectious score based on five parameters: symptoms from respiratory tract, ears and sinuses, malaise and antibiotic consumption.
The feeding protocol had no significant effect on ventilator-free days through 28 days (the primary endpoint), nor on infectious complications, ICU-free days, organ failure-free days or 60-day mortality.
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