Gradual reduction of mortality was observed with log-fold dilutions of the infectious dose (data not shown).
We found no evidence of any seasonal influence on the incidence rate of infectious conjunctivitis (data not shown).
The procedure of gel filtration of virus samples recovered 90 and 180 minutes after drying did not reduce the infectious titers (data not shown).
Likewise, release of adenoviruses from spots was not observed when the washing buffer was tested for infectious particles (data not shown).
In fact, American crows infected with Greece-10 (vs. the other strains used in this study) had the highest median peak viremia titers in terms of RNA and infectious virus (data not shown).
In animals infected SC with the sympatric enzootic strain Co97-0054 peak viremia levels of up to 3.3 log10PFU/mL developed 24 h postinfection, but the virus was undetectable by day 4. Similar viremia levels, and no detectable disease occurred in 3 spiny rats infected by the bite of an infectious mosquito (data not shown).
During the same period, no correlation was observed with tuberculosis, the other important mycobacterial disease in Victoria (r = 0.12, p = 0.43); legionellosis, caused by a nonvectored water-associated pathogen (r = 0.04, p = 0.66); or any other notifiable infectious disease (data not shown).
Moreover, infected histocultures released infectious virus particles (data not shown) suggesting that placental cells can support the entire lytic replication cycle.
Similarly, when HLA-DM, which is structurally similar to HLA-DR, was overexpressed in producer cells, Gag retention was also induced (Figure 4D) and infectious virus release reduced (data not shown).
Histopathologically, in the mice injected with the NIH230 strain, bacteria formed clusters in interstitial tissues in the kidneys and the lungs, accompanied congestion and no inflammatory cells at infectious foci (Figure 7B, 7C and data not shown).
