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This boosting correlated with a marked change in the impact of immune sera on MHV-68 infectivity: VAC-150 increased RAW264.7 cell infection, consistent with gp150 driving this process [21], but boosting with VAC-gB or VACgHL reduced it (Fig. 4A).
A sensitivity of 79.8 96.0% was demonstrated for Elecsys IgM in primary, early acute infection, consistent with existing assays.
Significantly higher proportions of females had a syphilis infection consistent with active (16% versus 6%, p<0.001) or lifetime infection (36% versus 12%, p<0.001) compared to males.
MyD88 also contributes significantly to survival of a primary pulmonary LVS infection, consistent with its reported role in the intradermal infection [33].
Protective antigen is so named for its ability to elicit a protective immune response to anthrax infection, consistent with evidence that protection provided by Biothrax™ vaccination is attributed to PA-specific antibodies [1], [4].
Additionally, IL12B expression is significantly decreased in patients with active TB compared to individuals with latent TB infection, consistent with the hypothesis that low expression of IFNγ in TB patients is due to the reduction of IL12-p40 [17]. IL12-p40 [17]
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Titres of infectious virus had increased ∼100-fold in supernatants from BJx109-, HKx31- and PR8-infected LA-4 cells between 2 and 24 hrs post-infection, consistent with productive infection of epithelial cells (data not shown).
This may depend on that 80 90% of these cells lack CD127 during primary HIV-infection, consistent with a predetermined apoptotic fate [39].
Bone marrows were hypercellular (Figure 2C), and increased splenic erythroid, myeloid and megakaryocytic EMH (Figure 3B) was observed at all time points post-infection, consistent with inflammation and murine responsive hematopoiesis [35], [38].
Lymphoid follicular disruption with depletion of the white pulp was also present (Figure 3B), particularly at six and 10 weeks post-infection, consistent with a previously described change in mice with marked EMH in response to anemia [36], and splenic pathology in people with HLH [28].
In the 10-week experiment (Table 1), bacterial colony forming units per gram (CFU/g) of liver and spleen was greatest at three weeks post-infection, consistent with the time-point of most severe HLH pathology (Tables 1 2), and decreased by six and 10 weeks post-infection.
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